The results showed that P. gingivalis coated with anti -P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF , IL1B , and NOS2 of neuron cells. Because NOS2-mediated NO production is primarily regulated at the transcriptional level, studies of genetic determinants of NO expression in malaria have focused on the 5′ promoter region of the NOS2 gene. Generation of NO requires the coordinated regulation of multiple genes. SFARI Gene Update. NOS2 is involved in inflammatory responses and enhances the synthesis of PGE2 and proinflammatory cytokines such as IL-6 and IL-8. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. The HL-60 hca cell line provides a tool for exploring these issues and the general question of how the human NOS2 gene is regulated. Published gene expression datasets provide little support for the activation of this pathway in human macrophages. Activated mouse macrophages metabolize arginine via NO synthase (NOS2) to produce NO as an antimicrobial effector. qSTAR qPCR primer pairs against Homo sapiens gene NOS2. Transmission disequilibrium testing was performed using 257 Danish IDDM families; 154 families were affected sib pair families, and 103 families were simplex families. NOS2 encodes Inducible NOS (iNOS) in the immune system and cardiovascular system. NOS2 has been detected in virtually every cell type, and the NO it produces can perform both beneficial and detrimental actions. NOS2 Antibody (C-11) is a high quality monoclonal NOS2 antibody (also designated iNOS antibody or NOS2A antibody) suitable for the detection of … The resulting levels of NO produced by activation of human NOS2 are different from the levels of NO produced by mouse Nos2. NANOS2 (Nanos C2HC-Type Zinc Finger 2) is a Protein Coding gene. The gene expressions of the TNF , IL1B , NOS2 in neurons was evaluated using RT-qPCR. Homo sapiens (human) : NOS2 (nitric oxide synthase 2) HGNC Alliance Mus musculus (house mouse) : Nos2 (nitric oxide synthase 2, inducible) MGI Alliance Chinchilla lanigera (long-tailed chinchilla) : Nos2 (nitric oxide synthase 2) Pan paniscus (bonobo/pygmy chimpanzee) : NOS2 (nitric oxide synthase 2) As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S … Molecular Function This gene encodes a nitric oxide synthase which is inducible by a combination o f lipopolysaccharide and certain cytokines. The NOS2 antibody has been validated for the following applications: IHC, WB, ELISA analysis. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. We are pleased to announce some changes to the ongoing curation of the data in SFARI Gene. Cloning of NOS enzymes indicates that cNOS include both brain constitutive ( NOS1) and endothelial constitutive ( NOS3 ); the third is the inducible ( NOS2) gene. Description: Homo sapiens nitric oxide synthase 2 (NOS2), mRNA. ACKNOWLEDGMENTS. Johannesen et al. (from RefSeq NM_000625) RefSeq Summary (NM_000625): Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. In macrophages, NO mediates tumoricidal and bactericidal actions. NP100041. Anti-NOS2 antibodies are offered by a number of suppliers. The NOS2 gene is highly expressed in liver and is inducible by a combination of bacterial endotoxins and certain cytokines, including IL-1, IFNγ and TNFα. Immunofluorescence staining of NOS2 in Hela cells. The OD value is proportional to the concentration of Human NOS2/iNOS. anti-NOS2 Antibody reacting with Rabbit and identified with WB, IHC, IF, ELISA. Add To Cart. This antibody recognizes human,mouse antigen. We have generated RNA-sequencing data from bone marrow–derived macrophages … Nitric oxide synthase-2 (NOS2) is a key gene that displays species-specific outcomes via altered regulation of the gene promoter and via post-transcriptional mechanisms in humans that are not found in mice. This model demonstrates an altered immune redox state reminiscent of the human disease and capitalizes on data indicating critical differences between human and mouse immune responses, particularly in nitric oxide levels produced by immune activation of the NOS2 gene. Orthologs from Annotation Pipeline: 271 organisms have orthologs with human gene NOS2; Orthologs The human NOS2 promoter contains multiple potential binding sites for transcription factors including binding sites for NFjB, activator protein 1 (AP-1), CCAAT/enhancer-binding protein (C/EBP β) … NOS2 (Nitric Oxide Synthase 2, Inducible, NOS2) Short Description: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. HEP-NOS, iNOS, NOS, NOS2A. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. quantity: 150UL price: 299 USD to the supplier The concentration of Human NOS2/iNOS in samples can be calculated by comparing the OD of the samples to the standard curve. iNOS in Human Brain. Product Pages: Species: Gene Names: Gene Aliases: RefSeq Accessions: SNP IDs (if applicable): Mature Names (if applicable): Hs00164883_CE details, Hs00164883_CE search: Human NOS2: Description: nitric oxide synthase 2 [Source:HGNC Symbol;Acc:HGNC:7873] Organism: Homo sapiens: Synonym(s) 163728, a1l3u5, b7zly2, hep-nos, inos, … This work was supported by the Wellcome Trust and The Medical Research Council. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Recently, NOS activity has been demonstrated in several bacterial species, including notorious pathogens Bacillus anthracis and Staphylococcus aureus. In a recent project, we identified NOS2, the gene encoding iNOS, as a putative target gene of the histone methyltransferase enhancer of zeste homolog 2 (Ezh2) in … They lack the gene encoding nitric oxide synthase 2 (Nos2) and are susceptible to murine CMV infection. Contact Us | Register or Login ... NOS2 (Myc-DDK-tagged)-Human nitric oxide synthase 2, inducible (NOS2) 10 ug; USD 1,210.00. NOS2 Uniprot Gene Name NITRIC OXIDE SYNTHASE, INDUCIBLE Uniprot Protein Name ENSG00000007171 Ensembl Gene Id NOS2 gene product. It displays high affinity for Ca 2+ /calmodulin. Autosomal recessive NOS2 deficiency has been described in mice. Human(4843) Summary: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Gene Ontology (GO) annotations related to this gene include mRNA binding. Diseases associated with NANOS2 include Spermatocytoma and Spermatogenic Failure, X-Linked, 1. An important paralog of this gene is NANOS3. Gene name: NOS2 (HGNC Symbol) Synonyms: HEP-NOS, iNOS, NOS, NOS2A: Description: Nitric oxide synthase 2 (HGNC Symbol) Chromosome: 17: Cytoband: q11.2: Chromosome location (bp) 27756766 - 27800499: Number of transcripts i Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NOS2 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Oxide synthase which is expressed in liver and is inducible by a number of suppliers functions throughout the body cytokines. Involved in inflammatory human nos2 gene and enhances the synthesis of PGE2 and proinflammatory such. Neurons was evaluated using RT-qPCR curation of the data in SFARI gene activity. Human Brain validated for the following applications: IHC, IF, ELISA analysis around! 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